As in Who Blacklisted Roger Rabbit contains a list of Toon cameos. In the interest of time and space, only the Toons that weren't in the first movie but are in the new one will be featured.
Walt Disney
Mickey Mouse
Minnie Mouse
Donald Duck
Daisy Duck
Goofy
Pluto
Pete
Horace Horsecollar
Clarabelle Cow
Huey, Dewey and Louie
Clara Cluck
Jose Carioca
Peter Pig
Mad Doctor
Professor Owl
Salty the Seal
Gus Goose
Chip 'n' Dale
Panchito Pistoles
Ludwig Von Drake
J. Audubon Woodlore
Humphrey the Bear
Lambert the Lion
Susie the Little Blue Coupe from Susie the Little Blue Coupe
Bucky Bug from Bugs in Love
Merry Dwarves from The Merry Dwarves
Trees and Flowers from Flowers and Trees
Sun from Father Noah's Ark
Big Bad Wolf, Three Little Pigs and Little Red Riding Hood
Toby Tortoise and Max Hare from The Tortoise and the Hare
Cock o' the Walk from Cock o' the Walk
Elmer Elephant and Joe Giraffe from Elmer Elephant
Snow White, Seven Dwarfs, Evil Queen (appearing as apple witch), One of the Vultures and Forest Animals from Snow White and the Seven Dwarfs
Ferdinand the Bull from Ferdinand the Bull
Pinocchio, Stromboli, Honest John and Gideon, Jiminy Cricket and Lampwick from Pinocchio
Brooms, Hyacinth Hippo, Madame Upanova, Ben Ali Gator, One of the Unicorns, Cupids and Chinese Mushrooms from Fantasia
Reluctant Dragon from The Reluctant Dragon
Dumbo, Timothy Q. Mouse, Crows, Mrs. Jumbo, Casey Junior and One of the Clowns from Dumbo
Thumper, Miss Bunny, Bambi, Great Prince, Faline and Flower from Bambi
Pedro the Plane from Saludos Amigos
Emotion from Reason & Emotion
Monte the Pelican from The Pelican and the Snipe
Chicken Little from Chicken Little
Flying Gauchito and Burrito from The Three Caballeros
Willy the Whale and Casey from Make Mine Music
Br'er Bear, Br'er Fox, Mr. Bluebird, Br'er Rabbit, Tar Baby, Hummingbirds and Sister Moles from Song of the South
Willie the Giant, Bongo, LuluBelle and Golden Harp from Fun and Fancy Free
Danny from So Dear to My Heart
Mr. Toad, Ichabod Crane and Cyril Proudbottom from The Adventures of Ichabod and Mr. Toad
Mr. Walker from Motor Mania
Alice, Mad Hatter, March Hare, Queen of Hearts, King of Hearts, White Rabbit, Card Soldiers, Dormouse, One of the Flamingos, Dodo, Bill the Lizard, Tweedledum and Tweedledee, Cheshire Cat and Doorknob from Alice in Wonderland
Apartments and Skyscrapers from The Little House
Witch Lezah from Trick or Treat
Peter Pan, Captain Hook, Mr. Smee, Wendy Darling, Lost Boys, Tiger Lily, Tick-Tock the Crocodile, Tinker Bell and John Darling from Peter Pan
Maleficent's Goons from Sleeping Beauty
Penguin Waiters and Butterflies from Mary Poppins
Flaps the Vulture and Kaa from The Jungle Book
Gopher from Winnie the Pooh Featurettes
Pongo (appearing as toy) and Perdita (appearing as toy) from One Hundred and One Dalmatians
Warner Bros.
Bugs Bunny
Daffy Duck
Elmer Fudd
Porky Pig
Yosemite Sam
Tweety
Sylvester
Foghorn Leghorn
Road Runner
Wile E. Coyote
Marvin the Martian
Sam Sheepdog
Speedy Gonzales
Marc Antony
Yoyo Dodo from Porky in Wackyland
Toro the Bull from Bully for Bugs
Pete Puma from Rabbit's Kin
Granny
Cecil Turtle
Witch Hazel
Pepe Le Pew
Beaky Buzzard
Barnyard Dawg
Hippity Hopper
Tasmanian Devil
Henery Hawk
Owl Jolson from I Love to Singa
Bosko
Gossamer
Minah Bird
MGM
Droopy
Screwy Squirrel
Meathead the Dog
Benny Burro
Barney Bear
George and Junior
Octopus from Half-Pint Pygmy
Tom and Jerry
Spike and Tyke
Quacker
Wolf from Red Hot Riding Hood
Turkey from Jerky Turkey
Paramount Pictures/Fleischer/Famous Studios
Betty Boop
Koko the Clown
Wiffle Piffle
Little Lulu
Joker (the harlequin jack-in-the-box logo for Noveltoons)
Popeye, Wimpy, Bluto and Olive Oyl
Little Audrey
Gabby
Casper the Friendly Ghost
Baby Huey
Herman and Katnip
Universal Studios/Walter Lantz
Woody Woodpecker
Wally Walrus
Buzz Buzzard
Andy Panda
Chilly Willy
Smedley
20th Century Fox Pictures/Terrytoons
Gandy Goose
Mighty Mouse
Heckle and Jeckle
King Features Syndicate/Pat Sullivan/Otto Soglow
Little King
Felix the Cat
Columbia Pictures/Screen Gems/UPA
The Fox and the Crow
Mr. Magoo
Winsor Mccay
Gertie the Dinosaur