After the end of the Green Goblin, Peter Parker continues to focus on things that are close to him, like his relationship with Gwen Stacy, and failed friendship with Harry Osborn, and his argument with Black Cat for leaving her father, Walter Hardy in prison, and he must team up with other heroes to fight off new and old enemies.
- Peter Parker/Spider-Man (Voiced by - Josh Keaton): Orphaned at a young age, Peter Parker was raised by his Aunt May and Uncle Ben as if he were their own son. Exceptionally bright but taunted by his peers, Peter's life was forever changed during a field trip to a since lab at Empire State University at the end of his sophomore year. While observing a lecture by Dr. Curt Connors, Peter was bitten on the hand by a genetically altered spider which had escaped from its container. The genetic code in the spider's venom rewrote Peter's DNA, giving him spider-related powers such as enhanced strength, enhanced agility, speed, reflexes, endurance, stamina, spider-sense and the ability to stick to almost any dry surface. Seeing potential in his powers, Peter crafted a pair of wrist-mounted web-shooters and a spider-themed costume before attending a wrestling match, under the name of Spider-Man, for a prize of $1,000. Despite quickly defeating the champion Crusher Hogan, Peter was denied his prize by the manager Sullivan Edwards unless he agreed to sign a contract. While leaving the match in anger and frustration, Peter chose not to intervene when he encountered a burglar (later identified as Walter Hardy) making off with money stolen from Edwards' vault, both out of spite for Edwards and indifferently believing it was not his responsibility. Upon reaching home, Peter was horrified to discover from his heartbroken aunt that a criminal had murdered Uncle Ben in cold blood for his car. Desperate for retribution against his uncle's killer, Peter donned his costume and web-shooters and cornered the criminal in a warehouse, but was stupefied to discover the culprit was the same man he had allowed to escape earlier that same day. While Peter was tempted to kill the burglar out of revenge, he knew uncle Ben wouldn't approve, so he left the criminal for the police to deal with. Filled with remorse for what his irresponsible choice cost him, Peter turned his Spider-Man persona into a superhero, finally taking to heart the message Uncle Ben tried to tell him the last time they spoke: "With great power, there must also come great responsibility."
- Harry Osborn/New Goblin (Voiced by - James Arnold Taylor)
- Gwen Stacy (Voiced by - Lacey Chabert)
- Mary Jane Watson (Voiced by - Vanessa Marshall)
- J. Jonah Jameson (Voiced by - Daran Norris)
- George Stacy (Voiced by - Clancy Brown)
- Liz Allan (Voiced by - Alanna Ubach)
- Glory Grant (Voiced by - Cree Summer)
- Sally Avril (Voiced by - Grey Delisle Griffin)
- Kenny Kong (Voiced by - Andrew Kishino)
- Ned Leeds (Voiced by - Andrew Kishino)
- Felicia Hardy/Black Cat (Voiced by - Tricia Helfer)
- Flash Thompson (Voiced by - Joshua LeBar)
- Dr. Curt Connors (Voiced by - Dee Bradley Baker)
- Martha Connors (Voiced by - Kath Souci)
- Betty Brant (Voiced by - Grey Delisle Griffin)
- Joseph "Robbie" Robertson (Voiced by - Kevin Michael Richardson)
- Randy "Rand" Robertson (Voiced by - Phil LaMarr)
- Hobie Brown/Prowler (Voiced by - Tim Russ)
- Sgt. Stan Carter (Voiced by - Thomas F. Wilson)
- Officer Jean DeWolff (Voiced by - Irene Bedard)
- Flint Marko/Sandman (Voiced by - John DiMaggio)
- Debra Whitman (Voiced by - Jacqueline Obradors)
- Fantastic Four:
- Johnny Storm/Human Torch (Voiced by - David Kaufman)
- Reed Richards/Mr. Fantastic (Voiced by - Cam Clarke)
- Sue Storm/Invisible Woman (Voiced by - Danica McKellar)
- Ben Grimm/Thing (Voiced by - John DiMaggio)
- Franklin Richards (Voiced by - Logan Grove)
- Matt Murdock/Daredevil (Voiced by - Brain Bloom)
- Robert "Robbie" Baldwin/Speedball (Voiced by - Benjamin Diskin)
- Bullseye (Voiced by - Wade Williams)
- Jessica Drew/Spider-Woman (Voiced by - Alyssa Milano)
- Kaine Parker (Voiced by - Josh Keaton)
- Danny Rand/Iron Fist (Voiced by - George Eads)
- Marc Spector/Moon Knight (Voiced by - Oded Fehr)
- Shang-Chi (Voiced by - Yuji Okimoto)
- Victor Von Doom/Doctor Doom (Voiced by - Tim Curry)
- Black Rose (Voiced by - Mark Rolston)
- Ben Urich (Voiced by - Michael T. Wiess)
- Carlie Cooper (Voiced by - Mae Whitman)
- Dum Dum Dugan (Voiced by - W. Morgan Sheppard)
- Philip DeWolff (Voiced by - Michael Horse)
- Celia DeWolff (Voiced by - Diane DeLano)
- Carl Weatherby (Voiced by - Robert Beltran)
- Brian DeWolff (Voiced by - Gregg Rainwater)
- Charles Rider (Voiced by - Adam Baldwin)
- Gloria Rider (Voiced by - Nicole Dubuc)
- Robert Rider (Voiced by - James Arnold Taylor)
- Lucia Von Bardas (Voiced by - Marina Sitris)
- Michele Gonzales (Voiced by - Kari Wahlgren)
- Luke Cage (Voiced by - John DiMaggio)
- Frank Castle/Punisher (Voiced by - Daran Norris)
- Chelsea Piers (Voiced by - Alyson Stoner)
- Green Goblin (Voiced by - Steven Blum)
- Norman Osborn (Voiced by - Alan Rachins)
- Green Goblin (Monster Form) (Voiced by - Dee Bradley Baker)
- Silvio Manfredi/Silvermane (Voiced by - Miguel Ferrer)
- Hobgoblin (Voiced by - Matt Frewer)
- Rodrick Kingsley (Voiced by - Courtney B. Vance)
- Miles Warren/Jackal (Voiced by - Brian George)
- John Jameson/Man-Wolf (voiced by Daran Norris)
- Eddie Brock/Venom (Voiced by - Benjamin Diskin)
- Cletus Kassidy/Carnage (Voiced by - Quinton Flynn)
- Dmitri Anatoly Nikolayevich Smerdyakov Kravinoff/Chameleon (Voiced by - Steven Blum)
- Hammerhead (Voiced by - John DiMaggio)
- Alex O'Hirn/Rhino (Voiced by - Clancy Brown)
- Quentin Beck/Mysterio (Voiced by - Xander Berkeley)
- Phineas Mason/Tinkerer (Voiced by - Thom Adcox)
- Otto Octavius/Dr. Octopus (Voiced by - Peter MacNicol)
- Sergei Kragonovff/Kraven The Hunter (Voiced by - Eric Vesbit)
- Montana/Shocker (Voiced by - Jeff Bennett)
- Mac Gargan/Scorpion (Voiced by - Kevin Grevouix)
- Adrian Toomes/Vulture (Voiced by - Robert Englund)
- Morris Bench/Hydro-Man (Voiced by - Bill Fagerbakke)
- Morbius (Voiced by - Steven Blum)
- Molten Man (Voiced by - Eric Lopez)
- L. Thompson Lincon/Tombstone (Voiced by - Dorian Harewood)
- Frederick Foswell/The Big Man/Patch (Voiced by - James Arnold Taylor)
- Max Dillon/Electro (Voiced by - Crispin Freeman)
- Wilson Fisk/Kingpin (Voiced by - David Sobolov)
- Marc Allan/Molten Man (Voiced by - Marc Lopez)
- Abner Jenkins/Beetle (Voiced by - Daran Norris)
- Jamie Thomason - Casting and Voice Director
- Sean "Cheeks" Galloway - Lead Character Designer
- Greg Weisman - Story Editor
Arc 1: New Heroes In Town
|27||Powerless, Part 1||April 1, 2017||
the first & second seasons.
|28||Powerless, Part 2||April 1, 2017||
Recaps: A Recap of the previous episode.
|29||Rivalry, Part 1||April 8, 2017||
Recaps: A Recap of Shear Strength, Subtext and Opening Night.
|30||Rivalry, Part 2||April 15, 2017||
Recaps: A Recap of Rivalry, Part 1, Opening Night & Reinforcement.
Arc 2: Secrets And Answers
|31||Bounty||April 22, 2017||
Recaps: A Recap of Reinforcement & Powerless.
|32||Lacking Skills||April 29, 2017||
Recaps: A Recap of Bounty.
|33||Cold Heart's Children||May 6, 2017||
Plot: Spider-Man and New Goblin team up with special guest star, Sly Cooper, who wants to help bring down the Children of Clockwerk and make up for lying to Inspector Carmelita Fox about his amnesia.
|34||Fighting for Favors||May 13, 2017||
Recaps: A Recap of Group Therapy and Powerless.
|35||Agent Spidey||May 20, 2017||
Recaps: A Recap of Cold Heart's Children
June 17, 2017
Recaps: A Recap of Cold Heart's Children, Fighting For Favors and Lacking Skills.
|37||Enemy of Interpol||June 24, 2017||
Recaps: A Recap of Cold Heart's Children and Dark Secrets.
|38||Expect The Unexpected||
July 1, 2017
Recaps: A Reacap of Growing Pains and Fighting For Favors.
|39||Tales of Suspense||
July 8, 2017
Recaps: A Recap of Intervention, Survival of the Fittest and Powerless.
Recaps: A Recap of Natural Selection, Fantastic Four, Tinkering the Wolf, Hobgoblin and Nothing to Fear.
Arc 3: Battle Against Crime
|41||New and Improved||
June 7, 2015
(Austrailia), July 22, 2015 (U.S.)
Recaps: A recap of Powerless and Fire and Ice.
June 11, 2015
(Austrailia), July 29, 2015 (U.S.)
Recaps: A recap of New and Improved.
|43||The Incredible Hulk||
June 12, 2015
(Austrailia), August 5, 2015 (U.S.)
Recaps: A recap of Group Therapy, Powerless, and Animal Rage.
|44||Scorpion's Sting||June 13, 2012 (Austrailia), August 19, 2012 (U.S.)||
Recaps: A Recap of Final Curtain, Powerless, Amazing Friendship and Tales to Astonish.
|45||Sand and Water||June 14, 2012 (Austrailia), September 9, 2012||
Recaps: A recap of First Steps and H2O.
|46||On Their Own||June 18, 2012 (Austrailia), September 16, 2012||
Recaps: A recap of Group Therapy, Powerless, Fantastic Four, Animal Rage, and Amazing Friendship.
|47||No Going Back||June 19, 2012 (Austrailia), September 23, 2012||
Recaps: A recap of Fantastic Four, Speedball, Animal Rage, Fire and Ice, Nothing to Fear, Tales of Suspense, Cloning, Amazing Friendship, Ghost Tales, The Incredible Hulk and Doomed.
Arc 4: The Final Stand
|48||Cut and Run Part 1||June 20, 2012 (Austrailia), September 30, 2012||
Recaps: A Recap of Nothing to Fear, Intervention, Accomplices, Probable Cause, Gangland and Opening Night.
|49||Cut and Run Part 2||June 21, 2012 (Austrailia), October 7, 2012||
Recaps: A Recap of Framed.
|50||Till All Are One Part 1: Take Off||June 25, 2012 (Austrailia), October 14, 2012||
Recaps: A Recap of Final Curtain, Scorpion's Sting and Hobgoblin.
|51||Till All Are One Part 2: Now or Never||June 26, 2012 (Austrailia), October 28, 2012||
Recaps: A Recap of Till All Are One
|52||Twelve vs Twelve Part 3: Endgame||June 27, 2012 (Austrailia), October 28, 2012||
Recaps: A Recap of Twelve vs Twelve