Supergirl: The Animated Series is an American computer animated television series based on DC comic's teenage heroine, Supergirl.
After leaving Argo City and arriving on earth. Supergirl is learning everything about earth
- Kara Zor-El/Linda Lee/Supergirl (voiced by Laura Bailey): The main protagonist. On Earth, Kara Supergirl adopts the secret identity of Linda Lee, a resident at Midvale Orphanage presided over by headmistress Miss Hartson. She disguises herself by concealing her blonde hair beneath a brunette wig. She's also a student who attends Midvale High.
- Ethan Malverin (voiced by Yuri Lowenthal): Kara's love interest
- Lucy Lane (voiced by Kristian Wigg): Kara's best friend and roommate.
- Nasthalthia "Natalie" Luthor (voiced by Emmanulle Chirqui): The main antagonist and the niece of Lex Luthor.
- Selena (Olivia Hack): Natalie's henchmen
- Donna Troy (Lacey Chabert):
- Clark Kent/Superman (voiced by George Newbern);
- Miss Hartson (voiced by Susan Blakeslee): Headmistriss
- Barbara Gordan/Batgirl (voiced by Ashley Johnson):
- Wendy Harris (Eddi McClurg):
- Marvin White (Alan Tudyk):
- Caitlin Fairchild ()
- Zor-El (voiced by Adam Baldwin):
- Legion of Super Heroes
- Lightning Lad
- Bouncing boy
- Chameleon Boy
- Saturn Girl
- Cosmic Boy
- A.T.L.A.S. (voiced by Mary Elizabeth McGlynn): A robot and A.I computer created by Lex Luthor's Niece to assist her with her plans destroy Supergirl.
- The Extremists:
- Lord Havok(Mark Oliver): Leader of the Extremists
- Dark Supergirl: Kara's split personality created by Black Kryptonite when Natalie Luthor used it on Kara.
- K-Force: A top-secret combat squadron designed for one specific purpose: to combat and defeat Supergirl, Wearing kryptonite-powered body armor.
- Conduit: Leader of K-Force
- Layla Adams/Sekhmen:
- Marc McGrath/Backlash: Welding a kryptonite energy whip and
- Deborah Morgna/Sungirl (Paget Brewster):
- Metallo (Paul Blackthorne):
- Parasite/Alexandra Allston (Natalie Chlugi)
- Brimstone (Kevin Grievoux):
- Cheshire (voiced by Kelly Hu)
- Mongul (voiced by Eric Roberts)
- Mongal (voiced by CCH Pounder)
- Atomic Spider (Vincent Tong): Chinese physicist Bo Hsu and his two assistants were working on one of the many nuclear projects for their government when something went very wrong. The three men's personalities and bodies were merged into one being, a being of great agility and empowered with immense nuclear might. The Atomic Spider is now a psychotic maniac, a wild card among the many super powered beings on the planet.
- Plant Man (Dave Wittenberg):
- Jaguar (Vikki Lewis) - similar to Cheetah, only she has the amulet of the Jaguar Spirit.
- Mad Mod - a former fashion designer, now I evil teacher super villain who captures and punishes superheroes for interfering with the plans of other villains, fighting crimes, and saving life.
- Maid of Might, Part 1:
- Maid of Might, Part 2: